Journal: iScience
Article Title: Ventral hippocampal cholecystokinin interneurons gate contextual reward memory
doi: 10.1016/j.isci.2024.108824
Figure Lengend Snippet:
Article Snippet: For immunostaining, free-floating brain sections were blocked with 5% normal donkey serum in 0.1% Triton X-100 in PBS (PBS-T) for 2 h. Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK-8 (1:1000, Sigma Millipore, C2581, rabbit anti-proCCK (Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000, Sigma Millipore, A2052), rabbit polyclonal anti-PV antibody (1:1000, Abcam, ab11427), rabbit polyclonal anti-vasoactive intestinal peptide (VIP) (1:500, Immunostar, 20077), rat polyclonal anti-somatostatin (1:500, Millipore, MAB354), rabbit polyclonal anti-cFos (1:1000, Santa Cruz Biotechnology), chicken polyclonal anti-GFP (1:1000, Abcam, ab13970), and goat polyclonal anti-mCherry (1:1000, Sicgen, AB0040-200).
Techniques: Virus, Plasmid Preparation, Recombinant, Software